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Upstream processing is the initial phase of the bioprocess from cell line development and cultivation to culture expansion of the cells through harvesting. Mammalian cell culture is an important tool for research, clinical, and pharmaceutical applications. In this course, students will learn techniques such as initiating, sub-culturing, trypsinization and cryopreserving a cell culture. Students will also have hands on experience performing cell counts, extracting media samples from a bioreactor and analyzing cell samples using a bio-analyzer to determine the growth rate of the cells.