Downstream processing is an intermediate phase that is in-between upstream/product harvest and final finish operations. The goal of this phase is to purify the product using multiple operations including cell disruption, filtration centrifuge, ultrafiltration, and chromatography. In this practical, students will perform a chromatography run with green fluorescent protein (GFP) using anion exchange chromatography (AEX).
- Beginners
- Downstream Processing
- Basic Lab Skills
- AEX Chromatography lab of GFP
Attendees who have completed this course should be able to:
- Explain the basics of biotechnology and the key biomanufacturing steps.
- Describe the goals of downstream processing. Explain the key operations and what they do including cell disruption, filtration, centrifugation, ultrafiltration and chromatography.
- Describe basic protein chemistry and its importance in chromatography operations. Explain protein denaturation and prevention.
- Describe the fundamentals of chromatography, types, operational steps, and performance testing.
- Perform the following steps for an anion-exchange chromatography for GFP: pH adjustment of filtrate, ID equipment, conditioning, performance test, GFP loading / eluting, sample analysis and cleanup. Demonstrate use of a UV/VIS spectrophotometer when performing analysis. Graph and complete the batch records.
Delivered in-person in a classroom or lab setting.
Downstream processing is an intermediate phase that is in-between upstream/product harvest and final finish operations. The goal of this phase is to purify the product using multiple operations including cell disruption, filtration centrifuge, ultrafiltration, and chromatography. In this practical, students will perform a chromatography run with green fluorescent protein (GFP) using anion exchange chromatography (AEX).
- Beginners
- Downstream Processing
- Basic Lab Skills
- AEX Chromatography lab of GFP
Attendees who have completed this course should be able to:
- Explain the basics of biotechnology and the key biomanufacturing steps.
- Describe the goals of downstream processing. Explain the key operations and what they do including cell disruption, filtration, centrifugation, ultrafiltration and chromatography.
- Describe basic protein chemistry and its importance in chromatography operations. Explain protein denaturation and prevention.
- Describe the fundamentals of chromatography, types, operational steps, and performance testing.
- Perform the following steps for an anion-exchange chromatography for GFP: pH adjustment of filtrate, ID equipment, conditioning, performance test, GFP loading / eluting, sample analysis and cleanup. Demonstrate use of a UV/VIS spectrophotometer when performing analysis. Graph and complete the batch records.
Delivered in-person in a classroom or lab setting.