Operations in Biotechnology Processes: An Overview

Operations in Biotechnology Processes: An Overview
850 Oval Drive
Raleigh, NC 27695


This course will focus on the details of the typical processing procedures utilized to produce a biopharmaceutical product. An understanding of the science and the equipment commonly used in each process step will be presented along with a review of the basic biochemistry involved in biotechnology and current pharmaceutical applications. Hands-on laboratory exercises will provide experience in the fermentation, product isolation, and purification of a protein.

Topics Include

This 4-day, 8-hour course covers the following topics:

  • Science of Biotechnology
  • Basic cGMP principles
  • Recombinant DNA techniques
  • Upstream Fermentation Processes
  • Brief history of fermentation, metabolism and growth
  • Fermentation principles,equipment, controls and monitoring
  • Bioreactor component identification
  • Gene Expression Systems
  • Chemistry of Proteins
  • Downstream Processes
  • Principles of Chromatography (Size exclusion, affinity, ionic exchange, hydrophobic interaction)
  • Supernatant for chromatography preparation
  • Chromatography system component identification
  • Ultrafiltration, Evaporation, Precipitation, Liquid extraction and Crystallization
  • Cell Disruption
  • Electrophoresis

Learning Objectives

  • Explain the fundamental science behind biotechnology and its application in the pharmaceutical industry.
  • Discuss basic science, equipment, and processing steps typically utilized in the production, isolation and purification of a biopharmaceutical product.
  • Demonstrate proficiency with common instrumentation used in the production, isolation and purification of a biopharmaceutical product.


This course is designed for entry level process technicians, incumbent workers as well as experienced professionals in Manufacturing, Quality Management or Senior Management positions in the biopharmaceutical industry.

David Yarley
Class Dates
  • April 16, 2018
  • April 17, 2018
  • April 18, 2018
  • April 19, 2018
Class Days & Times
  • Mon - 8:00 AM to 5:00 PM
  • Tue - 8:00 AM to 5:00 PM
  • Wed - 8:00 AM to 5:00 PM
  • Thu - 8:00 AM to 5:00 PM
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