High performance liquid chromatography (HPLC) is a chemistry technique used to separate compounds of interest from a liquid mixture based on chemical and/or physical properties. When HPLC is coupled with a spectrophotometric detector, it can be used to quantify analytes to provide detailed information on the chemical composition of a sample. HPLC is commonly used in quality control or research laboratories in the biotechnology, chemical, agricultural, pharmaceutical, dietary supplement, and cosmetic industries. This course will focus on the basic applications of HPLC, including an introduction to separation science, detection methods, and the varieties of chromatography modes via HPLC. Additional topics include instrument orientation and operation, maintenance and troubleshooting, method development, sample preparation, and data analysis. Laboratory sessions will include hands-on experience in calibration of an HPLC system.