Counting Cells with a Hemocytometer

Description

There can be tens of thousands of cells in one milliliter of culture medium. So how are cells counted?

The process requires diluting the cell culture, dying the cells, and loading the cells into a hemocytometer. A hemocytometer is a modified microscope slide with two chambers. Each chamber is divided into 9 squares. There are specific guidelines for counting cells within the squares.

Share

Current Courses
View All
May
8
Fundamentals of Microbiology
Learn how to apply fundamental microbiological techniques to a wide range of manufacturing processes regulated by the FDA, USDA, and other agencies. In this course, you’ll delve into concepts and techniques of microbial detection, isolation, enumeration, and identification plus examine written
June
25
Aseptic Processing I
Are you new to the pharmaceutical or life science  sectors? Need to brush up your skills? Learn and practice the basics of aseptic operations in a simulated CGMP environment. CGMP exists to protect the health and safety of patients or consumers. Aseptic Processing Level I provides the foundational
Current Courses
View All
May
8
Fundamentals of Microbiology
Learn how to apply fundamental microbiological techniques to a wide range of manufacturing processes regulated by the FDA, USDA, and other agencies. In this course, you’ll delve into concepts and techniques of microbial detection, isolation, enumeration, and identification plus examine written
June
25
Aseptic Processing I
Are you new to the pharmaceutical or life science  sectors? Need to brush up your skills? Learn and practice the basics of aseptic operations in a simulated CGMP environment. CGMP exists to protect the health and safety of patients or consumers. Aseptic Processing Level I provides the foundational
eLearning
View All
Virtual Microscope
Virtual Microscope
BioNetwork’s Virtual Microscope is the first fully interactive 3D scope - it’s a great practice tool to prepare you for working in a science lab. Learn terminology, usage, and care by interacting with a fully functional, virtual microscope. When you're ready, challenge your knowledge in the testing
Virtual Microscope Companion
Virtual Microscope Companion
The Virtual Microscope Companion provides a self-guided exploration of a common laboratory microscope including its care, components, and usage. Learners can practice and test their knowledge in preparation for working with a real microscope. 
eLearning
View All
Virtual Microscope
Virtual Microscope
BioNetwork’s Virtual Microscope is the first fully interactive 3D scope - it’s a great practice tool to prepare you for working in a science lab. Learn terminology, usage, and care by interacting with a fully functional, virtual microscope. When you're ready, challenge your knowledge in the testing
Virtual Microscope Companion
Virtual Microscope Companion
The Virtual Microscope Companion provides a self-guided exploration of a common laboratory microscope including its care, components, and usage. Learners can practice and test their knowledge in preparation for working with a real microscope. 
eLearning
View All
Virtual Microscope
Virtual Microscope
BioNetwork’s Virtual Microscope is the first fully interactive 3D scope - it’s a great practice tool to prepare you for working in a science lab. Learn terminology, usage, and care by interacting with a fully functional, virtual microscope. When you're ready, challenge your knowledge in the testing
Virtual Microscope Companion
Virtual Microscope Companion
The Virtual Microscope Companion provides a self-guided exploration of a common laboratory microscope including its care, components, and usage. Learners can practice and test their knowledge in preparation for working with a real microscope. 
Videos
View All
Use and Care of a Microscope
Use and Care of a Microscope
Learn how to use a microscope, see it in action, and then head to the lab to practice working hands-on. In this video, Clarence (our rotifer microscopy expert), describes the functions and procedures for using a microscope including: Stage controls Coarse and fine focus and more...
Microscope for Beginners - Questions and Answers
Microscope for Beginners - Q&A
In this video, Jeff talks through some common issues and questions that beginners experience when learning to use a microscope.  We'll discuss:  Common mistakes Keeping both eyes open Artifacts or specimens Finding and centering the specimen Oil immersion - how it works Cleaning 
BioNetwork Capstone Center
Capstone Center
Capstone Center in Raleigh is BioNetwork's premiere worker training facility.  At Capstone, fundamental scientific understanding meets hands-on application.  Our industry-experienced instructors lead courses in bioprocessing, quality control, compliance, cGMP, analytical chemistry, and microbiology
  •  
  • 1 of 2
  • >
Videos
View All
Use and Care of a Microscope
Use and Care of a Microscope
Learn how to use a microscope, see it in action, and then head to the lab to practice working hands-on. In this video, Clarence (our rotifer microscopy expert), describes the functions and procedures for using a microscope including: Stage controls Coarse and fine focus and more...
Microscope for Beginners - Questions and Answers
Microscope for Beginners - Q&A
In this video, Jeff talks through some common issues and questions that beginners experience when learning to use a microscope.  We'll discuss:  Common mistakes Keeping both eyes open Artifacts or specimens Finding and centering the specimen Oil immersion - how it works Cleaning 
BioNetwork Capstone Center
Capstone Center
Capstone Center in Raleigh is BioNetwork's premiere worker training facility.  At Capstone, fundamental scientific understanding meets hands-on application.  Our industry-experienced instructors lead courses in bioprocessing, quality control, compliance, cGMP, analytical chemistry, and microbiology
  •  
  • 1 of 2
  • >
Videos
View All
BioNetwork Capstone Center
Capstone Center
Capstone Center in Raleigh is BioNetwork's premiere worker training facility.  At Capstone, fundamental scientific understanding meets hands-on application.  Our industry-experienced instructors lead courses in bioprocessing, quality control, compliance, cGMP, analytical chemistry, and microbiology
Applying a Simple Stain to a Bacterial Culture
Applying a Simple Stain to a Bacterial Culture
Applying a simple stain to a bacterial culture is a technique that is used for examining the size, shape, and arrangement of a specimen. It uses a dye to stain the cells, making them easier to see under a microscope. In this experiment, we will demonstrate the steps for applying a simple stain to
Use and Care of a Microscope
Use and Care of a Microscope
Learn how to use a microscope, see it in action, and then head to the lab to practice working hands-on. In this video, Clarence (our rotifer microscopy expert), describes the functions and procedures for using a microscope including: Stage controls Coarse and fine focus and more...
  •  
  • 1 of 2
  • >
Activities
View All
Piguliar Investigation
A PIGuliar Investigation
Zoonosis (a disease that can be transmitted to humans from animals) in swine are increasingly becoming a global public health concern. In this activity students will be introduced to a swine influenza outbreak, the potential dangers it poses to the human population, and perform electrophoresis of
Restriction Digestion and Analysis of Lambda DNA
Restriction Digestion and Analysis of Lambda DNA
Electrophoretic techniques that distinguish DNA fragments by size are essential in forensics and in the mapping of restriction sites within genes. With the Restriction Digestion and Analysis of Lambda DNA Kit, students use three different restriction enzymes to digest genomic DNA from lambda
Sore Throat Scrutiny
Sore Throat Scrutiny
Explore the significance of antibody-antigen interaction and its role in immunoprecipitation testing. Determine if a patient's sore throat is a symptom of a bacterial infection such as strep throat or a viral infection. (Note: Requires several hours of room temperature incubation for results to
Activities
View All
Piguliar Investigation
A PIGuliar Investigation
Zoonosis (a disease that can be transmitted to humans from animals) in swine are increasingly becoming a global public health concern. In this activity students will be introduced to a swine influenza outbreak, the potential dangers it poses to the human population, and perform electrophoresis of
Restriction Digestion and Analysis of Lambda DNA
Restriction Digestion and Analysis of Lambda DNA
Electrophoretic techniques that distinguish DNA fragments by size are essential in forensics and in the mapping of restriction sites within genes. With the Restriction Digestion and Analysis of Lambda DNA Kit, students use three different restriction enzymes to digest genomic DNA from lambda
Sore Throat Scrutiny
Sore Throat Scrutiny
Explore the significance of antibody-antigen interaction and its role in immunoprecipitation testing. Determine if a patient's sore throat is a symptom of a bacterial infection such as strep throat or a viral infection. (Note: Requires several hours of room temperature incubation for results to
Activities
View All
Restriction Digestion and Analysis of Lambda DNA
Restriction Digestion and Analysis of Lambda DNA
Electrophoretic techniques that distinguish DNA fragments by size are essential in forensics and in the mapping of restriction sites within genes. With the Restriction Digestion and Analysis of Lambda DNA Kit, students use three different restriction enzymes to digest genomic DNA from lambda
Sore Throat Scrutiny
Sore Throat Scrutiny
Explore the significance of antibody-antigen interaction and its role in immunoprecipitation testing. Determine if a patient's sore throat is a symptom of a bacterial infection such as strep throat or a viral infection. (Note: Requires several hours of room temperature incubation for results to
Piguliar Investigation
A PIGuliar Investigation
Zoonosis (a disease that can be transmitted to humans from animals) in swine are increasingly becoming a global public health concern. In this activity students will be introduced to a swine influenza outbreak, the potential dangers it poses to the human population, and perform electrophoresis of