Sample Preparation for HPLC Analysis

This course must be taught at a BioNetwork location.

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Sample Preparation for HPLC Analysis
Level: Intermediate
Lecture Hours: 8.00
Lab Hours: 8.00
Total Contact Hours: 16.00


Gain hands-on experience in sample preparation and purification techniques. You will learn to analyze biological samples for trace levels of drug and pesticide residues using HPLC (High Performance Liquid Chromatography).

Students perform standard preparation, reagent preparation, sample extractions, and typical purification procedures.

Participants also learn about calibrating an HPLC system as well learn to follow a High Performance Liquid Chromatography method developed to determine trace levels of an organic contaminant in milk samples.

Topics Include

  • Basics of High Performance Liquid Chromatography (HPLC)
  • Use of top loading and analytical balances, pipettes, and volumetric flasks
  • Introduction to bio-analytical (trace organic) analysis
  • Sampling, sample extraction, and purification techniques,  including solid phase extraction and solvent partitioning techniques
  • Standard solution preparations
  • HPLC auto injection of calibration standards on an overnight run
  • Calibration of an HPLC unit
  • Importance of lab controls and lab-fortified controls as quality control checks
  • Demonstration of Method M 002 to determine a trace-level organic contaminant in milk
  • Challenges posed by real world samples

Learning Objectives

  • Demonstrate competency in extracting trace samples from complex biological matrices.
  • Execute basic analytical processes and use HPLC instrumentation to perform trace organic analysis.


We designed this course for experienced professionals, including:

  • Laboratory Technician
  • Analytical Chemist
  • HPLC Scientist
  • Associate Scientist
  • Quality Control Analyst
  • Research and Development Personnel